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Combined use of mitochondrial and nuclear genetic markers further reveal immature marine turtle hybrids along the South Western Atlantic Genet. Mol. Biol.
Brito,Cíntia; Vilaça,Sibelle Torres; Lacerda,Ana Luzia; Maggioni,Rodrigo; Marcovaldi,Maria Ângela; Vélez-Rubio,Gabriela; Proietti,Maíra Carneiro.
Abstract Marine turtle hybridization is usually sporadic and involves reports of only a few individuals; however, Brazilian populations have high hybridization rates. Here we investigated the presence of hybrids in morphologically identified immature hawksbills (Eretmochelys imbricata) along the South Western Atlantic (SWA). We sequenced one mitochondrial (D-Loop) and three nuclear DNA (RAG1, RAG2, and CMOS) markers to better understand the patterns and characteristics of hybrids. We identified 22 hybrids (n = 270), 11 of them at the extreme South of the SWA. Uruguay had the highest hybrid frequency in the SWA (~37.5%) followed by southern Brazil with 30%. These are common areas for loggerheads (Caretta caretta) but uncommon for hawksbills, and these...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hybridization; Cheloniidae; Genetic markers; Hybrid distribution; Conservation.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000400704
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DNA sampling from eggshells and microsatellite genotyping in rare tropical birds: Case study on Brazilian Merganser Genet. Mol. Biol.
Maia,Thais Augusta; Vilaça,Sibelle Torres; Silva,Luciana Resende da; Santos,Fabricio Rodrigues; Dantas,Gisele Pires de Mendonça.
Abstract This study shows that sampling maternal DNA from hatched and abandoned eggshells is a viable noninvasive strategy for studying the genetics of rare or endangered tropical birds, as exemplified here by the Brazilian Merganser (Mergus octosetaceus). Eighteen microsatellites were isolated from enriched libraries and nine heterologous loci from related species were tested. Seven loci were amplified successfully, with five of them being polymorphic. These loci exhibited amplicons ranging from 110 to 254 bp for 132 samples, with 60 from eggshells and 72 from blood or muscle samples. The number of alleles for M. octosetaceus ranged from one to six (mean = 3.71), which is low compared to M. merganser (1-15 alleles), a ‘least concern’ species. Genetic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: DNA; Noninvasive samples; Genetic diversity; Endangered birds; Mergus.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000500808
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